解剖学报

›› 2010, Vol. 41 ›› Issue (5): 761-763.doi: 10.3969/j.issn.0529-1356.2010.05.024

• 论著 • 上一篇    下一篇

人胚胎干细胞系chHES-20表达生殖细胞分化相关基因

彭翠英1,2;卢光琇1*   

  1. 1. 中南大学生殖与干细胞工程研究所人类干细胞国家工程中心，长沙410078; 2. 南华大学药学与生命科学学院，湖南 衡阳421001
  • 收稿日期:2010-03-29 修回日期:2010-05-05 出版日期:2010-10-06
  • 通讯作者: 卢光琇

Human embryonic stem cells line chHES-20 expresses germ cell differentiation associated genes

  1. 1. Institute of Reproduction and Stem Cell Engineering, Central South University，National Engineering Research Center of Human Stem Cell，Changsha410078, China; 2. Pharmacy and Life Science, South China University, Hu’nan Hengyang421001, China
  • Received:2010-03-29 Revised:2010-05-05 Online:2010-10-06
  • Contact: LU Guang-xiu

关键词: 胚胎干细胞, 生殖细胞, 分化, 基因, 反转录-聚合酶链反应, 人

Abstract: Objective To investigate whether the human embryonic stem cell line chHES-20 can express the germ cell differentiation associated genes. Methods RT-PCR was used to detect the expression pattern of germ cell differentiation associated genes in chHES-20 cell line. The present sdudy used the normal human testis and human embryonic fibroblast cell line to serve as the positive and negative controls, respectively. Results The pluripotency and self-renewal specific genes OCT4,NANOG and SOX2 were able to be expressed in chHES-20 cell line.The DAZL(deleted in azoospermia-like) and FRAGILIS of pre-meiotic germ cell-specific genes were expressed in chHES-20 cell line. chHES-20 was not able to express the meiosis specific gene SCP3(synapto complex protein 3 gene) and germ cell pos-meiotic differentiation gene VASA. Conclusion chHES-20 embryonic stem cell line possesses the potential to differentiate germ cell.

Key words: Embryonic stem cell, Germ cell, Differentiation, Gene, RT-PCR, Human

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